LI ZHOU | 30 Nov 06:04 2013
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Re: problem with bp_genbank2gff.pl

Hi,
Thank you for your answers.
I am now trying to install the latest version (BioPerl-1.6.922) on another computer. 
I need to install some missing modules. But theres are some errors when I try to install GD and GraphViz.
The following are the error message:

>perl Build installdeps
Checking optional dependencies:
Install GD? [y] y
Install GraphViz? [y] y
CPAN: Storable loaded ok (v2.41)
Reading '/Users/zhouli/.cpan/Metadata'
  Database was generated on Wed, 13 Nov 2013 01:17:02 GMT
Running install for module 'GD'
Running make for L/LD/LDS/GD-2.50.tar.gz
CPAN: Digest::SHA loaded ok (v5.85)
CPAN: Compress::Zlib loaded ok (v2.063)
Checksum for /Users/zhouli/.cpan/sources/authors/id/L/LD/LDS/GD-2.50.tar.gz ok
CPAN: File::Temp loaded ok (v0.2304)
CPAN: Parse::CPAN::Meta loaded ok (v1.4409)
CPAN: CPAN::Meta loaded ok (v2.132830)
CPAN: Module::CoreList loaded ok (v2.96)

  CPAN.pm: Building L/LD/LDS/GD-2.50.tar.gz

Notice: Type perl Makefile.PL -h for command-line option summary.

**UNRECOVERABLE ERROR**
Could not find gdlib-config in the search path. Please install libgd 2.0.28 or higher.
If you want to try to compile anyway, please rerun this script with the option --ignore_missing_gd.
Warning: No success on command[/Users/zhouli/perl5/perlbrew/perls/perl-5.18.1/bin/perl Makefile.PL]
CPAN: YAML loaded ok (v0.84)
  LDS/GD-2.50.tar.gz
  /Users/zhouli/perl5/perlbrew/perls/perl-5.18.1/bin/perl Makefile.PL -- NOT OK
Running make test
  Make had some problems, won't test
Running make install
  Make had some problems, won't install
Could not read metadata file. Falling back to other methods to determine prerequisites
Running install for module 'GraphViz'
Running make for R/RS/RSAVAGE/GraphViz-2.14.tgz
Checksum for /Users/zhouli/.cpan/sources/authors/id/R/RS/RSAVAGE/GraphViz-2.14.tgz ok
CPAN: Module::Build loaded ok (v0.42)

  CPAN.pm: Building R/RS/RSAVAGE/GraphViz-2.14.tgz

Please install Graphviz from http://www.graphviz.org/.
Warning: No success on command[/Users/zhouli/perl5/perlbrew/perls/perl-5.18.1/bin/perl Build.PL]
  RSAVAGE/GraphViz-2.14.tgz
  /Users/zhouli/perl5/perlbrew/perls/perl-5.18.1/bin/perl Build.PL -- NOT OK
Running Build test
  Make had some problems, won't test
Running Build install
  Make had some problems, won't install

I am new to computational biology. I would appreciate if you could offer any help.
Thank you very much!
Regards,
Zhou Li

On 30 Nov, 2013, at 12:55 PM, Scott Cain <scott <at> scottcain.net> wrote:

> Also, about your questions (which were buried very deep at the end of the email):
> 
> 1. The script supports a --split option to split GFF from fasta. 
> 
> 2. I don't think there is an option to specifically skip attributes like translation (it shows up in the GFF
because it's in the genbank file), but it probably wouldn't be to hard to add that functionality. Patches
welcome. 
> 
> 3. I don't know anything about igv. 
> 
> Scott
> 
> 
> Sent from my iPhone
> 
> On Nov 29, 2013, at 3:34 PM, "Fields, Christopher J" <cjfields <at> illinois.edu> wrote:
> 
>> The latest CPAN release (v 1.6.922) fixes these issues.
>> 
>> chris
>> 
>> On Nov 28, 2013, at 1:08 AM, zhou li <zhouli <at> tll.org.sg<mailto:zhouli <at> tll.org.sg>> wrote:
>> 
>> Dear Bioperl people,
>> I am using BioPerl-1.6.1, and the operating system is Mac OS X version 10.8.5.
>> I am trying to convert a local GenBank file to GFF file using bp_genbank2gff.pl, using the following command,
>> $ bp_genbank2gff.pl M21017.gb --stdout > M21017.gff3
>> And I got the following message, I am not sure if this is an error:
>> Replacement list is longer than search list at
/Users/zhouli/perl5/perlbrew/perls/perl-5.18.1/lib/site_perl/5.18.1/Bio/Range.pm line 251.
>> UNIVERSAL->import is deprecated and will be removed in a future perl at
/Users/zhouli/perl5/perlbrew/perls/perl-5.18.1/lib/site_perl/5.18.1/Bio/Tree/TreeFunctionsI.pm
line 94.
>> # working on region:M21017, Drosophila melanogaster, 09-MAY-1994, D.melanogaster 18S, 5.8S 2S and
28S rRNA genes, complete, and 18S rRNA gene, 5' end, clone pDm238.
>> 
>> ***************************************************************************
>> And the output file M21017.gff3 is attached.
>> 
>> $head M21017.gff3
>> ##gff-version 3
>> M21017 Genbank region 1 12026 . . . ID=M21017;Note=D.melanogaster%2018S%2C%205.8S%202S%20and%2028S%20rRNA%20genes%2C%20complete%2C%20and%2018S%20rRNA%20gene%2C%205%27%20end%2C%20clone%20pDm238.;Alias=M29800
>> M21017 Genbank region 1 12026 . + . ID=Drosophila%20melanogaster;db_xref=taxon%3A7227;mol_type=genomic%20DNA
>> M21017 Genbank gene 1 12026 . + . ID=18S%20rRNA
>> M21017 Genbank RNA 1 7232 . + . ID=18S%20rRNA;note=rRNA%20primary%20transcript
>> M21017 Genbank rRNA 1 1995 . + . ID=18S%20rRNA;product=18S%20ribosomal%20RNA
>> M21017 Genbank gene 2722 2844 . + . ID=5.8S%20rRNA
>> M21017 Genbank rRNA 2722 2844 . + . ID=5.8S%20rRNA;product=5.8S%20ribosomal%20RNA
>> M21017 Genbank gene 2873 2902 . + . ID=2S%20rRNA
>> M21017 Genbank rRNA 2873 2902 . + . ID=2S%20rRNA;product=2S%20ribosomal%20RNA
>> 
>> 
>> When I test another genbank file
>> $ bp_genbank2gff.pl WSSV-AF369029-GenBank.gb --stdout > WSSV-AF369029-GenBank.gff3
>> I also got the error message:
>> Replacement list is longer than search list at
/Users/zhouli/perl5/perlbrew/perls/perl-5.18.1/lib/site_perl/5.18.1/Bio/Range.pm line 251.
>> UNIVERSAL->import is deprecated and will be removed in a future perl at
/Users/zhouli/perl5/perlbrew/perls/perl-5.18.1/lib/site_perl/5.18.1/Bio/Tree/TreeFunctionsI.pm
line 94.
>> $ head WSSV-AF369029-GenBank.gff3
>> ##gff-version 3
>> AF369029 Genbank region 1 292967 . . . ID=AF369029;Alias=AY864671;Note=White%20spot%20syndrome%20virus%2C%20complete%20genome.
>> AF369029 Genbank region 1 292967 . + . ID=White%20spot%20syndrome%20virus;mol_type=genomic%20DNA;isolate=WSSV-TH;country=Thailand;db_xref=taxon%3A342409
>> AF369029 Genbank gene 1 615 . + . ID=VP28;experiment=experimental%20evidence%2C%20no%20additional%20details%20recorded;note=envelope%20protein
>> AF369029 Genbank CDS 1 615 . + . Parent=VP28.t00;translation=MDLSFTLSVVSAILAITAVIAVFIVIFRYHNTVTKTIETHTDNIETNMDENLRIPVTAEVGSGYFKMTDVSFDSDTLGKIKIRNGKSDAQMKEEDADLVITPVEGRALEVTVGQNLTFEGTFKVWNNTSRKINITGMQMVPKINPSKAFVGSSNTSSFTPVSIDEDEVGTFVCGTTFGAPIAATAGGNLFDMYVHVTYSGTETE;db_xref=GI%3A15021393;protein_id=AAK77670.1;product=ORF1%2C%20VP28%2C%20gene%20family%201;note=envelope%20protein;codon_start=1
>> AF369029 Genbank CDS 710 2902 . - . Parent=AAK77671.1.t00;translation=MEGGDQRTKLTPATVMGLYQSKTPGEGEGGEGGGQFKIPSAIAVKSCCSKNATRRSPPSDSPYSLRPMKRLKKNNGEVGGKAPPPVTLRLREDYESTPYNFNRNKKKRPITIDENQFATLNPTYATDIIKKQQLPSVSAASVLRKHRANADTQYRKRFSHPNCAKFSTVNLKARDYTPLSVLRSHVKGPKHLKSSCDTVTETNVVKRNFSSIDKWVKLEKPPCYFAVAEADTNIAAGLESPFHLIRQAAKLGLISDVQDVSSNYETIKQSCIDAKEKASKFLWSNNRTKQPPSSWWPVGFGSKNLSVLDTSPLLNWNRLCKNNGKGWIKTMSIDHMAKNVFKLSPGACESILEKKTTLLGEVTAQCKKWESYRRNIPVPAHVQPEYASQVVMIGPSELYLEVKVGVYYMLETGKVIKFMTDKEMYCEFVFETVFSHALEGRMKGAVGVRKMCVEGFCVEMDFAGISVIDVLNGDLKCKMDENVVQQPNPSTTSSKPAAELMQDHGSLCRMRDTLYGVRMLQATGRLPEGLQSKCKKPITDSISAIAIVGKMRERMLNQLPFVLVEIVNIVTRLSQQGLVNPDIKSDNIVIDGITGQPKMIDFGLIVPCKKYYNFKCWGTDERFFSNHPHTAPEFINSELCSETAMTFGLAYLLIDMLSILIKRTADLSANSIYTNIPFLSIVSKMYDQEKTNRPRAYEIAPVIGACFPFKDNIAKLFQSPKHSLYSK
 KVK;db_xref=GI%3A15021394;codon_start=1;product=ORF2%2C%20putative%20serine%2Fthreonine%20protein%20kinase%20%28PK1%29%2C%20gene%20family%202
>> AF369029 Genbank CDS 3118 4989 . - . Parent=AAK77672.1.t00;codon_start=1;product=ORF3;db_xref=GI%3A15021395;translation=MAWTVMALKDAFTERLVVNKVGSGTDMAPVVEDDRQKSLFQKVENLYRVLVVEQKNSAITLSGNKNTNKRQCRQVEEDKVIFEGEDRTVSNLPQAVKETIAANAESILDYWYKNVIPLLDTKKERSGKSDTFLRTAVICLVRCCVSYKDMKTCSLIYEFEHKILNKSTLDPLLKDILDNKQELLHMDSKYGSKTTSPELAKETIEALYTTVYNHWTNAFKLYQASLTHKPVTGKKYASVIHFIRTWRKIVKAYVSKHNNVERDLSLKNIMKNESADNANVLTIEKMYKKIGNSVKNTNNNSAHQMSDSEDDDDDDDDDCEGMDVCDEASEREKKHQESLYPINTPVTTITGDYIFKVLLELVLSPHIHPEWKIPMCDFVNRNIPKLMKAMETDISNAVIEVRASKVNPVQILPIAANFWDFCKSGKPPSDVKFCMMFNEPSSNETLSSGAGVFGRFIGGPFSHKSKELDIISNCLRSLLLNKEADNLSTRIWREGGSVVCFNYCPITARGAVLGYGEQLSERSIKALWAKKIQDAVTESVKRQRNAADKNSRNCDLLGDEGVVSMKTVTFGCANMLKTQNGMGKFNVVVSFEDSIQANKEGAARQYMSQQVFTHSFPALDQGK
>> 
>> The output file is so tedious, the translation is all showing up. But to me, it is not needed.
>> 1. Is there any way to make the output file more succinct without having the translation included?
>> 2. Also, is there any way to split the output file to two files, one is the GFF3 file and the other one is DNA
fasta sequence file?
>> 3. When I import the WSSV-AF369029-GenBank.gff3 file to IGV, it displays the protein ID if there is no
gene name for the sequence, e.g. those with feature "CDS" display the protein ID, and those with feature
"gene" display gene ID, is this the way it works? I want to display the ORF ID, what should I do?
>> 
>> 
>> 
>> [TLL Conference]<http://conference.tll.org.sg/>
>> 
>> TLL is organizing an international conference on Next Generation Genomic
>> View on Plants, Animals and Microbes on March 5th to 7th, 2014. For more
>> information, please visit http://conference.tll.org.sg<http://conference.tll.org.sg/>
>> --------------------------------------------------------------------------------
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>> please notify the sender, and please delete the message and any other record of it
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>> 
>> 
>> Your help is greatly appreciated.
>> Thank you very much!
>> Regards,
>> Zhou Li
>> 
>> 
>> 
>> 
>> <PastedGraphic-1.pdf><M21017.gff3><WSSV-AF369029-GenBank.gff3>
>> 
>> 
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