6 Oct 2012 00:31
issue with Views on PairwiseAlignmentsSingleSubject?
Hi there,
I've been using Biostrings' pairwiseAlignment for various things. I just saw the Views feature described
in help("PairwiseAlignments-class") and thought I'd give it a try, but it's not behaving as I'd expect in
cases where my subject sequence is one sequence selected from a DNAStringSet (rather than starting with a DNAString).
I think I found a bug (?) although I could also be misunderstanding what Views is supposed to do. I updated to
the new Bioc devel and new R today, but had the same issue before updating.
I think the code chunk below will explain - does it make sense?
thanks very much,
Janet
library(Biostrings)
## example seqs (taken from ?pairwiseAlignment)
s1 <- DNAString("ACTTCACCAGCTCCCTGGCGGTAAGTTGATCAAAGGAAACGCAAAGTTTTCAAG")
s2 <- DNAString("GTTTCACTACTTCCTTTCGGGTAAGTAAATATATAAATATATAAAAATATAATTTTCATC")
### make some sequence sets
s1a <- DNAStringSet(c(as.character(s1),as.character(s1)))
s2a <- DNAStringSet(c(as.character(s2),as.character(s2)))
### align
myAln <- pairwiseAlignment ( s1a[1], s2a[1])
### I think the next line of code should give me a Views on s1a[1], but instead it gives me Views on all the seqs
of s1a concatenated together
Views(myAln)
##### and, an aside, it would seem intuitive to me that the code below should work to make a stringset of two
sequences, but instead it concetenates them to one sequence
s1b <- DNAStringSet(c(s1,s1))
#########
sessionInfo()
R Under development (unstable) (2012-10-03 r60868)
Platform: x86_64-unknown-linux-gnu (64-bit)
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=C LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] Biostrings_2.27.1 IRanges_1.17.0 BiocGenerics_0.5.0
loaded via a namespace (and not attached):
[1] parallel_2.16.0 stats4_2.16.0
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