4 Dec 2012 23:46
HT-seq counting - gene vs isoform
Hi, I am trying to understand the counting done by HTseq using Ensemble GTF file. Here is an example: GeneA_isoform1: Exon1 Exon2 Exon3 GeneA_isoform2: Exon1 Exon3 GeneA_isoform3:Exon1 Exon4 When counting at gene level, I assume the reads that fall on all exons (Exon1, Exon2, Exon3 and Exon4) are all summed up for GeneA. When counting at isoform level, GeneA_isoform1 - is it sum of exons from Exon1, Exon2 and Exon3 (or) just reads that map to Exon2? GeneA_isoform2 - is it sum of Exon1 and Exon3 (or) no counts because its exons are common with isoform1 and isoform3? GeneA_isoform3 - sum of Exon1 and Exon4 (or) only Exon4? Thank you very much for the clarification. Best Regards, Nirmala ------------------------------------------------------------------------------------------------------------------------------ Contractor Buiding 35, Room 1A-205 35 Convent Drive, National Institute of Mental Health/NIH Bethesda MD - 20892 Phone# 301-451-4258 [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list Bioconductor@... https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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