13 Feb 2013 23:38
find gene symbols immediately flanking before and after a SNP position
Dear all, I have a list of several hundred SNP that I would like to annotate functionally and am able to do this via websites such as SeattleSeq. However, for intergenic SNPs it does not give me the neighbouring genes. Therefore, I have tried to find genes immediately flanking a SNP (one left and right) in R. I note that this question has been asked previously. I am trying to follow one of the previous suggestions (https://stat.ethz.ch/pipermail/bioconductor/2010-December/037185.html). I been struggling with this for the last two days but I think I am getting something fundamentally wrong. I have chosen the following two SNPs (among several thousands). I am expecting to see the following kind of output: rs881375 (chr9:123652898) is located between PHF19 and TRAF1 rs12191877 (chr6:31252925) is located between RPL3P2 and WASF5P First, I code the query up as a GRange object: rsid <- c("rs881375", "rs12191877") chr <- c("chr9", "chr6") pos <- c(123652898, 31252925) library(GenomicFeatures) target <- GRanges( seqnames = Rle( chr ), ranges = IRanges(pos, end=pos, names=rsid), strand = Rle(strand( rep("*", length(chr)) )) ) # GRanges with 2 ranges and 0 metadata columns: # seqnames ranges strand # <Rle> <IRanges> <Rle> # rs881375 chr9 [123652898, 123652898] * # rs12191877 chr6 [ 31252925, 31252925] * # --- # seqlengths: # chr6 chr9 # NA NA txdb <- makeTranscriptDbFromUCSC("hg19") # about 5 min tx <- transcriptsBy(txdb) But when I try precede( target, tx ) follow( target, tx ) I get the following message: Error in function (classes, fdef, mtable) : unable to find an inherited method for function ‘precede’ for signature ‘"GRanges", "GRangesList"’ Any help would be very much appreciated. I am happy to try other packages or websites if available. Many thanks. Regards, Adai _______________________________________________ Bioconductor mailing list Bioconductor@... https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
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